PCR Multiplexing Based on a Single Fluorescent Channel Using Dynamic Melting Curve Analysis
نویسندگان
چکیده
منابع مشابه
Single-channel multiplexing without melting curve analysis in real-time PCR
Multiplex real-time PCR with quantification of targets in a single fluorescence channel has been the demand in biotechnology industry. Here, we develop a novel analytical real-time PCR technique to detect multiple targets in a single fluorescence channel without melting curve analysis. In this technique, we show the intensity of the fluorescence signals of two discrete Tm targets is different a...
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ژورنال
عنوان ژورنال: ACS Omega
سال: 2020
ISSN: 2470-1343,2470-1343
DOI: 10.1021/acsomega.0c04766